prikaz prve stranice dokumenta Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
  Preuzmi
PDF 736.05 KB
Znanstveni rad - Izvorni znanstveni rad
Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
Scientific Reports, 12 (2022), 1; 1237. https://doi.org/10.1038/s41598-022-05315-7
Rodriguez-Temporal, David; Alcaide, Fernando; Mareković, Ivana; O’Connor, James Anthony; Gorton, Rebecca; van Ingen, Jakko; Van den Bossche, An; Héry-Arnaud, Genevieve; Beauruelle, Clémence; Orth-Höller, Dorothea; Palacios-Gutiérrez, Juan-José; Tudó, Griselda; Bou, Germán; Ceyssens, Pieter-Jan; Garrigó, Montserrat; González-Martin, Julià; Greub, Gilbert; Hrabak, Jaroslav; Ingebretsen, André; Mediavilla-Gradolph, Maria Concepción; Oviaño, Marina; Palop, Begoña; Pranada, Arthur B.; Quiroga, Lidia; Ruiz-Serrano, Maria Jesús; Rodríguez-Sánchez, Belén Više autora...

Citirajte ovaj rad

Rodriguez Temporal, D., Alcaide, F., Mareković, I., O’Connor, J. A., Gorton, R., van Ingen, J. ... Rodríguez Sánchez, B. (2022). Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports, 12. (1). doi: 10.1038/s41598-022-05315-7

Rodriguez Temporal, David, et al. "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification." Scientific Reports, vol. 12, br. 1, 2022. https://doi.org/10.1038/s41598-022-05315-7

Rodriguez Temporal, David, Fernando Alcaide, Ivana Mareković, James Anthony O’Connor, Rebecca Gorton, Jakko van Ingen, An Van den Bossche, et al. "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification." Scientific Reports 12, br. 1 (2022). https://doi.org/10.1038/s41598-022-05315-7

Rodriguez Temporal, D., et al. (2022) 'Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification', Scientific Reports, 12(1). doi: 10.1038/s41598-022-05315-7

Rodriguez Temporal D, Alcaide F, Mareković I, O’Connor JA, Gorton R, van Ingen J, i sur.. Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification. Scientific Reports [Internet]. 24.01.2022. [pristupljeno 08.11.2024.];12(1). doi: 10.1038/s41598-022-05315-7

D. Rodriguez Temporal, et al., "Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification", Scientific Reports, vol. 12, br. 1, Siječanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:313006. [Citirano: 08.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:313006
Prijavite se u repozitorij kako biste mogli spremiti objekt u svoju listu.
Podaci o radu
Naslov (engleski)Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification
AutorDavid Rodriguez-Temporal
AutorFernando Alcaide
AutorIvana Mareković
AutorJames Anthony O’Connor
AutorRebecca Gorton
AutorJakko van Ingen
AutorAn Van den Bossche
AutorGenevieve Héry-Arnaud
AutorClémence Beauruelle
AutorDorothea Orth-Höller
AutorJuan-José Palacios-Gutiérrez
AutorGriselda Tudó
AutorGermán Bou
AutorPieter-Jan Ceyssens
AutorMontserrat Garrigó
AutorJulià González-Martin
AutorGilbert Greub
AutorJaroslav Hrabak
AutorAndré Ingebretsen
AutorMaria Concepción Mediavilla-Gradolph
AutorMarina Oviaño
AutorBegoña Palop
AutorArthur B. Pranada
AutorLidia Quiroga
AutorMaria Jesús Ruiz-Serrano
AutorBelén Rodríguez-Sánchez
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU MIKROBIOLOGIJU I PARAZITOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on the reproducibility of MALDI-TOF have been performed so far, none on mycobacteria. The aim of this study was to evaluate the reproducibility of MALDI-TOF for the identification of NTM in 15 laboratories in 9 European countries. A total of 98 NTM clinical isolates were grown on Löwenstein-Jensen. Biomass was collected in tubes with water and ethanol, anonymized and sent out to the 15 participating laboratories. Isolates were identified using MALDI Biotyper (Bruker Daltonics). Up to 1330 MALDI-TOF identifications were collected in the study. A score ≥ 1.6 was obtained for 100% of isolates in 5 laboratories (68.2–98.6% in the other). Species-level identification provided by MALDI-TOF was 100% correct in 8 centres and 100% correct to complex-level in 12 laboratories. In most cases, the misidentifications obtained were associated with closely related species. The variability observed for a few isolates could be due to variations in the protein extraction procedure or to MALDI-TOF system status in each centre. In conclusion, MALDI-TOF showed to be a highly reproducible method and suitable for its implementation for NTM identification.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaScientific Reports
Brojčani podacivol. 12, br. 1, 1237
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-022-05315-7
URN:NBNurn:nbn:hr:105:313006
Datum objave publikacije2022-01-24
URL dokumentahttps://www.nature.com/articles/s41598-022-05315-7
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2022-11-14 12:35:18