prikaz prve stranice dokumenta Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions
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Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions
Frontiers in Immunology, 13 (2022); 902947. https://doi.org/10.3389/fimmu.2022.902947
Filipović, Maša; Flegar, Darja; Šućur, Alan; Šisl, Dino; Kavazović, Inga; Antica, Mariastefania; Kelava, Tomislav; Kovačić, Nataša; Grčević, Danka

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Filipović, M., Flegar, D., Šućur, A., Šisl, D., Kavazović, I., Antica, M. ... Grčević, D. (2022). Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions. Frontiers in Immunology, 13.. doi: 10.3389/fimmu.2022.902947

Filipović, Maša, et al. "Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions." Frontiers in Immunology, vol. 13, 2022. https://doi.org/10.3389/fimmu.2022.902947

Filipović, Maša, Darja Flegar, Alan Šućur, Dino Šisl, Inga Kavazović, Mariastefania Antica, Tomislav Kelava, Nataša Kovačić i Danka Grčević. "Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions." Frontiers in Immunology 13 (2022). https://doi.org/10.3389/fimmu.2022.902947

Filipović, M., et al. (2022) 'Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions', Frontiers in Immunology, 13. doi: 10.3389/fimmu.2022.902947

Filipović M, Flegar D, Šućur A, Šisl D, Kavazović I, Antica M, i sur.. Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions. Frontiers in Immunology [Internet]. 2022. [pristupljeno 09.11.2024.];13. doi: 10.3389/fimmu.2022.902947

M. Filipović, et al., "Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions", Frontiers in Immunology, vol. 13, 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:115400. [Citirano: 09.11.2024.]

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Naslov (engleski)Inhibition of Notch Signaling Stimulates Osteoclastogenesis From the Common Trilineage Progenitor Under Inflammatory Conditions
AutorMaša Filipović
AutorDarja Flegar
AutorAlan Šućur
AutorDino Šisl
AutorInga Kavazović
AutorMariastefania Antica
AutorTomislav Kelava
AutorNataša Kovačić
AutorDanka Grčević
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA ANATOMIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA FIZIOLOGIJU I IMUNOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Anatomija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Fiziologija čovjeka
Sažetak (engleski)
Osteoclasts, macrophages and dendritic cells (DCs) can be derived from a common trilineage myeloid progenitor of hematopoietic origin. Progenitor commitment is susceptible to regulation through Notch signaling. Our aim was to determine the effects of Notch modulation on trilineage progenitor commitment and functional properties of differentiated cells under inflammatory conditions. We used the conditional inducible CX3CR1CreERT2 mouse strain to achieve overexpression of the Notch 1 intracellular domain (NICD1) or to inhibit Notch signaling via deletion of the transcription factor RBP-J in a bone marrow population, used as a source of the trilineage progenitor (CD45+Ly6G-CD3-B220-NK1.1-CD11b-/loCD115+). Cre-recombinase, under the control of the CX3CR1 promoter, expressed in the monocyte/macrophage lineage, was induced in vitro by 4-hydroxytamoxifen. Differentiation of osteoclasts was induced by M-CSF/RANKL; macrophages by M-CSF; DCs by IL-4/GM-CSF, and inflammation by LPS. Functionally, DCs were tested for the ability to process and present antigen, macrophages to phagocytose E. coli particles, and osteoclasts to resorb bone and express tartrate-resistant acid phosphatase (TRAP). We found that Notch 1 signal activation suppressed osteoclast formation, whereas disruption of the Notch canonical pathway enhanced osteoclastogenesis, resulting in a higher number and size of osteoclasts. RANK protein and Ctsk gene expression were upregulated in osteoclastogenic cultures from RBP-J+ mice, with the opposing results in NICD1+ mice. Notch modulation did not affect the number of in vitro differentiated macrophages and DCs. However, RBP-J deletion stimulated Il12b and Cd86 expression in macrophages and DCs, respectively. Functional assays under inflammatory conditions confirmed that Notch silencing amplifies TRAP expression by osteoclasts, whereas the enhanced phagocytosis by macrophages was observed in both NICD1+ and RBP-J+ strains. Finally, antigen presentation by LPS-stimulated DCs was significantly downregulated with NICD1 overexpression. This experimental setting allowed us to define a cell-autonomous response to Notch signaling at the trilineage progenitor stage. Although Notch signaling modulation affected the activity of all three lineages, the major effect was observed in osteoclasts, resulting in enhanced differentiation and function with inhibition of canonical Notch signaling. Our results indicate that Notch signaling participates as the negative regulator of osteoclast activity during inflammation, which may be relevant in immune and bone diseases.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaFrontiers in Immunology
Brojčani podacivol. 13, 902947
e-ISSN1664-3224
DOIhttps://doi.org/10.3389/fimmu.2022.902947
URN:NBNurn:nbn:hr:105:115400
Datum objave publikacije2022
URL dokumentahttps://www.frontiersin.org/articles/10.3389/fimmu.2022.902947/full
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-03-01 17:02:27