prikaz prve stranice dokumenta 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
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4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
International Journal of Molecular Sciences, 23 (2022), 22; 14373. https://doi.org/10.3390/ijms232214373
Cindrić, Marina; Čipak Gašparović, Ana; Milković, Lidija; Tartaro Bujak, Ivana; Mihaljević, Branka; Žarković, Neven; Žarković, Kamelija

Citirajte ovaj rad

Cindrić, M., Čipak Gašparović, A., Milković, L., Tartaro Bujak, I., Mihaljević, B., Žarković, N. i Žarković, K. (2022). 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes. International Journal of Molecular Sciences, 23. (22). doi: 10.3390/ijms232214373

Cindrić, Marina, et al. "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes." International Journal of Molecular Sciences, vol. 23, br. 22, 2022. https://doi.org/10.3390/ijms232214373

Cindrić, Marina, Ana Čipak Gašparović, Lidija Milković, Ivana Tartaro Bujak, Branka Mihaljević, Neven Žarković i Kamelija Žarković. "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes." International Journal of Molecular Sciences 23, br. 22 (2022). https://doi.org/10.3390/ijms232214373

Cindrić, M., et al. (2022) '4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes', International Journal of Molecular Sciences, 23(22). doi: 10.3390/ijms232214373

Cindrić M, Čipak Gašparović A, Milković L, Tartaro Bujak I, Mihaljević B, Žarković N, i sur.. 4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes. International Journal of Molecular Sciences [Internet]. 19.11.2022. [pristupljeno 15.04.2024.];23(22). doi: 10.3390/ijms232214373

M. Cindrić, et al., "4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes", International Journal of Molecular Sciences, vol. 23, br. 22, Studeni 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:170700. [Citirano: 15.04.2024.]

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Naslov (engleski)4-Hydroxynonenal Modulates Blood–Brain Barrier Permeability In Vitro through Changes in Lipid Composition and Oxidative Status of Endothelial Cells and Astrocytes
AutorMarina Cindrić
AutorAna Čipak Gašparović
AutorLidija Milković
AutorIvana Tartaro Bujak
AutorBranka Mihaljević
AutorNeven Žarković
AutorKamelija Žarković
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Patologija
Sažetak (engleski)
Blood brain barrier (BBB) is a dynamic interface responsible for proper functioning of brain, but also a major obstacle for effective treatment of neurological diseases. Increased levels of free radicals, in high ferrous and high lipid content surrounding, induce lipid peroxidation, leading to production of 4-hydroxynonenal (HNE). HNE modifies all key proteins responsible for proper brain functioning thus playing a major role in the onset of neurological diseases. To investigate HNE effects on BBB permeability, we developed two in vitro BBB models–‘physiological’ and ‘pathological’. The latter mimicked HNE modified extracellular matrix under oxidative stress conditions in brain pathologies. We showed that exogenous HNE induce activation of antioxidative defense systems by increasing catalase activity and glutathione content as well as reducing lipid peroxide levels in endothelial cells and astrocytes of ‘physiological’ model. While in ‘pathological’ model, exogenous HNE further increased lipid peroxidation levels of endothelial cells and astrocytes, followed by increase in Nrf2 and glutathione levels in endothelial cells. At lipid composition level, HNE caused increase in ω3 polyunsaturated fatty acid (PUFA) level in endothelial cells, followed by decrease in ω3 PUFA level and increase in monounsaturated fatty acid level in astrocytes. Using these models, we showed for the first time that HNE in ‘pathological’ model can reduce BBB permeability.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 23, br. 22, 14373
e-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms232214373
URN:NBNurn:nbn:hr:105:170700
Datum objave publikacije2022-11-19
ProjektŠifra: 108-0000000-0028
Naziv (hrvatski): Oksidacijski stres i tumori središnjeg živčanog sustava
Naziv (engleski): Oxidative stress and central nervous system tumors
Voditelj: Kamelija Žarković
Pravna nadležnost: Hrvatska
Financijer: MZOS
Linija financiranja: ZP
ProjektŠifra: 098-0982464-2519
Naziv (hrvatski): Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji
Naziv (engleski): Lipids, free radicals and their messengers in integrative oncology
Voditelj: Neven Žarković
Pravna nadležnost: Hrvatska
Financijer: MZOS
Linija financiranja: ZP
URL dokumentahttps://www.mdpi.com/1422-0067/23/22/14373
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-06-13 11:02:43