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Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
Life, 12 (2022), 11; 1744. https://doi.org/10.3390/life12111744
Perše, Gabrijela; Samošćanec, Ivana; Bošnjak, Zrinka; Budimir, Ana; Kuliš, Tomislav; Mareković, Ivana

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Perše, G., Samošćanec, I., Bošnjak, Z., Budimir, A., Kuliš, T. i Mareković, I. (2022). Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life, 12. (11). doi: 10.3390/life12111744

Perše, Gabrijela, et al. "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry." Life, vol. 12, br. 11, 2022. https://doi.org/10.3390/life12111744

Perše, Gabrijela, Ivana Samošćanec, Zrinka Bošnjak, Ana Budimir, Tomislav Kuliš i Ivana Mareković. "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry." Life 12, br. 11 (2022). https://doi.org/10.3390/life12111744

Perše, G., et al. (2022) 'Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry', Life, 12(11). doi: 10.3390/life12111744

Perše G, Samošćanec I, Bošnjak Z, Budimir A, Kuliš T, Mareković I. Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry. Life [Internet]. 30.10.2022. [pristupljeno 22.04.2024.];12(11). doi: 10.3390/life12111744

G. Perše, I. Samošćanec, Z. Bošnjak, A. Budimir, T. Kuliš i I. Mareković, "Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry", Life, vol. 12, br. 11, Listopad 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:998321. [Citirano: 22.04.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:998321
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Podaci o radu
Naslov (engleski)Sepsityper® Kit versus In-House Method in Rapid Identification of Bacteria from Positive Blood Cultures by MALDI-TOF Mass Spectrometry
AutorGabrijela Perše
AutorIvana Samošćanec
AutorZrinka Bošnjak
MBZ: 319631
AutorAna Budimir
AutorTomislav Kuliš
AutorIvana Mareković
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU MIKROBIOLOGIJU I PARAZITOLOGIJU)
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA UROLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Urologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
In order to further accelerate pathogen identification from positive blood cultures (BC), various sample preparation protocols to identify bacteria with MALDI-TOF MS directly from positive BCs have been developed. We evaluated an in-house method in comparison to the Sepsityper® Kit (Bruker Daltonics, Bremen, Germany) as well as the benefit of an on-plate formic acid extraction step following positive signal by the BACTECTM FX system. Confirmation of identification was achieved using subcultured growing biomass used for MALDI-TOF MS analysis. A total of 113 monomicrobial positive BCs were analyzed. The rates of Gram-positive bacteria correctly identified to the genus level using in-house method and Sepsityper® Kit were 63.3% (38/60) and 81.7% (49/60), respectively (p = 0.025). Identification rates at species level for Gram-positive bacteria with in-house method and Sepsityper® kit were 30.0% (18/60) and 66.7% (40/60), respectively (p < 0.001). Identification rates of Gram-negative bacteria were similar with the in-house method and Sepsityper® Kit. Additional on-plate formic acid extraction demonstrated significant improvement in the identification rate of Gram-positive bacteria at both genus and species level for both in-house (p = 0.001, p < 0.001) and Sepsityper® Kit methods (p = 0.007, p < 0.001). Our in-house method is a candidate for laboratory routines with Sepsityper® Kit as a back-up solution when identification of Gram-positive bacteria is unsuccessful.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaLife
Brojčani podacivol. 12, br. 11, 1744
e-ISSN2075-1729
DOIhttps://doi.org/10.3390/life12111744
URN:NBNurn:nbn:hr:105:998321
Datum objave publikacije2022-10-30
URL dokumentahttps://www.mdpi.com/2075-1729/12/11/1744
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-07-13 06:53:26