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Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
Antibodies, 12 (2023), 2; 35. https://doi.org/10.3390/antib12020035
Vilibić-Čavlek, Tatjana; Bogdanić, Maja; Borko, Ema; Hruskar, Zeljka; Zilic, Denis; Ferenc, Thomas; Tabain, Irena; Barbić, Ljubo; Vujica Ferenc, Mateja; Ferenčak, Ivana; Stevanović, Vladimir

Citirajte ovaj rad

Vilibić Čavlek, T., Bogdanić, M., Borko, E., Hruskar, Z., Zilic, D., Ferenc, T. ... Stevanović, V. (2023). Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test. Antibodies, 12. (2). doi: 10.3390/antib12020035

Vilibić Čavlek, Tatjana, et al. "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test." Antibodies, vol. 12, br. 2, 2023. https://doi.org/10.3390/antib12020035

Vilibić Čavlek, Tatjana, Maja Bogdanić, Ema Borko, Zeljka Hruskar, Denis Zilic, Thomas Ferenc, Irena Tabain, et al. "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test." Antibodies 12, br. 2 (2023). https://doi.org/10.3390/antib12020035

Vilibić Čavlek, T., et al. (2023) 'Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test', Antibodies, 12(2). doi: 10.3390/antib12020035

Vilibić Čavlek T, Bogdanić M, Borko E, Hruskar Z, Zilic D, Ferenc T, i sur.. Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test. Antibodies [Internet]. 10.05.2023. [pristupljeno 11.04.2024.];12(2). doi: 10.3390/antib12020035

T. Vilibić Čavlek, et al., "Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test", Antibodies, vol. 12, br. 2, Svibanj 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:669514. [Citirano: 11.04.2024.]

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Podaci o radu
Naslov (engleski)Detection of SARS-CoV-2 Antibodies: Comparison of Enzyme Immunoassay, Surrogate Neutralization and Virus Neutralization Test
AutorTatjana Vilibić-Čavlek
AutorMaja Bogdanić
AutorEma Borko
AutorZeljka Hruskar
AutorDenis Zilic
AutorThomas Ferenc
AutorIrena Tabain
AutorLjubo Barbić
AutorMateja Vujica Ferenc
AutorIvana Ferenčak
AutorVladimir Stevanović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA MEDICINSKU MIKROBIOLOGIJU I PARAZITOLOGIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
Background: Since sensitivity and specificity vary widely between tests, SARS-CoV-2 serology results should be interpreted with caution. Methods: The study included serum samples from patients who had recovered from COVID-19 (n = 71), individuals vaccinated against SARS-CoV-2 (n = 84), and asymptomatic individuals (n = 33). All samples were tested for the presence of binding antibodies (enzyme immunoassay; EIA), neutralizing (NT) antibodies (virus neutralization test; VNT), and surrogate NT (sNT) antibodies (surrogate virus neutralization test; sVNT) of SARS-CoV-2. Results: SARS-CoV-2-binding antibodies were detected in 71 (100%) COVID-19 patients, 77 (91.6%) vaccinated individuals, and 4 (12.1%) control subjects. Among EIA-positive samples, VNT was positive (titer ≥ 8) in 100% of COVID-19 patients and 63 (75.0%) of the vaccinated individuals, while sVNT was positive (>30% inhibition) in 62 (87.3%) patients and 59 (70.2%) vaccinated individuals. The analysis of antibody levels showed a significant moderate positive correlation between EIA and VNT, a moderate positive correlation between EIA and sVNT, and a strong positive correlation between VNT and sVNT. The proportion of positive sVNT detection rate was associated with VNT titer. The lowest positivity (72.4%/70.8%) was detected in samples with low NT titers (8/16) and increased progressively from 88.2% in samples with titer 32 to 100% in samples with titer 256. Conclusions: sVNT appeared to be a reliable method for the assessment COVID-19 serology in patients with high antibody levels, while false-negative results were frequently observed in patients with low NT titers.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaAntibodies
Brojčani podacivol. 12, br. 2, 35
e-ISSN2073-4468
DOIhttps://doi.org/10.3390/antib12020035
URN:NBNurn:nbn:hr:105:669514
Datum objave publikacije2023-05-10
URL dokumentahttps://www.mdpi.com/2073-4468/12/2/35
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2023-10-06 08:10:11