Botulinum toxin's axonal transport from periphery to the spinal cord

Znanstveni rad - Izvorni znanstveni rad

• Autor: Ivica Matak
• Autor: Peter Riederer
• Autor: Zdravko Lacković
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti

Sažetak (engleski)

Axonal transport of enzymatically active botulinum toxin A (BTX-A) from periphery to the CNS has been described in facial and trigeminal nerve, leading to cleavage of synaptosomal-associated protein 25 (SNAP-25) in central nuclei. Aim of present study was to examine the existence of axonal transport of peripherally applied BTX-A to spinal cord via sciatic nerve. We employed BTX-A-cleaved SNAP-25 immunohistochemistry of lumbar spinal cord after intramuscular and subcutaneous hind limb injections, and intraneural BTX-A sciatic nerve injections. Truncated SNAP-25 in ipsilateral spinal cord ventral horns and dorsal horns appeared after single peripheral BTX-A administrations, even at low intramuscular dose applied (5 U/kg). Cleaved SNAP-25 appearance in the spinal cord after BTX-A injection into the sciatic nerve was prevented by proximal intrasciatic injection of colchicine (5 mM, 2 μl). Cleaved SNAP-25 in ventral horn, using choline-acetyltransferase (ChAT) double labeling, was localized within cholinergic neurons. These results extend the recent findings on BTX-A retrograde axonal transport in facial and trigeminal nerve. Appearance of truncated SNAP-25 in spinal cord following low-dose peripheral BTX-A suggest that the axonal transport of BTX-A occurs commonly following peripheral application.

• Ključne riječi (engleski): Animals; Axonal Transport/physiology; Botulinum Toxins, Type A/administration & dosage; Botulinum Toxins, Type A/metabolism; Choline O-Acetyltransferase/metabolism; Colchicine/pharmacology; Hindlimb/physiology; Immunohistochemistry; Injections; Injections, Intramuscular; Injections, Subcutaneous; Male; Microscopy, Confocal; Motor Neurons/metabolism; Peripheral Nerves/metabolism; Rats; Rats, Wistar; Sciatic Nerve/metabolism; Sensory Receptor Cells/metabolism; Spinal Cord/metabolism; Synaptosomal-Associated Protein 25/metabolism
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Završna verzija rukopisa prihvaćena za objavljivanje (postprint)
• Naslov časopisa: Neurochemistry International
• Brojčani podaci: vol. 61, br. 2, str. 236-239
• p-ISSN: 0197-0186
• DOI: https://doi.org/10.1016/j.neuint.2012.05.001
• URN:NBN: urn:nbn:hr:105:651272
• Datum objave publikacije: 2012-07
• URL dokumenta: https://doi.org/10.1016/j.neuint.2012.05.001
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-27 19:34:56