Stable gastric pentadecapeptide BPC 157 heals cysteamine-colitis and colon-colon-anastomosis and counteracts cuprizone brain injuries and motor disability

Znanstveni rad - Izvorni znanstveni rad

• Autor: Robert Kliček
• Autor: Danijela Kolenc
• Autor: Jelena Šuran
• Autor: Domagoj Drmić
• Autor: Luka Brčić
• Autor: Gorana Aralica
• Autor: Marko Sever
• Autor: J. Holjevac
• Autor: Božo Radić
• Autor: Tanja Turudić
• Autor: A. Kokot
• Autor: Leonardo Patrlj
• Autor: Rudolf Rucman
• Autor: Sven Seiwerth
• Autor: Predrag Sikirić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti

Sažetak (engleski)

Stable gastric pentadecapeptide BPC 157 was suggested to link inflammatory bowel disease and multiple sclerosis, and thereby, shown to equally counteract the models of both of those diseases. For colitis, cysteamine (400 mg/kg intrarectally (1 ml/rat)) and colon-colon anastomosis (sacrifice at day 3, 5, 7, and 14) were used. BPC 157 (10 μg/kg, 10 ng/kg) was applied either intraperitoneally once time daily (first application immediately after surgery, last at 24 hours before sacrifice) or per-orally in drinking water (0.16 μg/ml/12 ml/day till the sacrifice) while controls simultaneously received an equivolume of saline (5 ml/kg) intraperitoneally or drinking water only (12 ml/day). A multiple sclerosis suited toxic rat model, cuprizone (compared with standard, a several times higher regimen, 2.5% of diet regimen + 1 g/kg intragastrically/day) was combined with BPC 157 (in drinking water 0.16 μg or 0.16 ng/ml/12 ml/day/rat + 10 μg or 10 ng/kg intragastrically/day) till the sacrifice at day 4. In general, the controls could not heal cysteamine colitis and colon-colon anastomosis. BPC 157 induced an efficient healing of both at the same time. Likewise, cuprizone-controls clearly exhibited an exaggerated and accelerated damaging process; nerve damage appeared in various brain areas, with most prominent damage in corpus callosum, laterodorsal thalamus, nucleus reunions, anterior horn motor neurons. BPC 157-cuprizone rats had consistently less nerve damage in all damaged areas, especially in those areas that otherwise were most affected. Consistently, BPC 157 counteracted cerebellar ataxia and impaired forelimb function. Thereby, this experimental evidence advocates BPC 157 in both inflammatory bowel disease and multiple sclerosis therapy.

• Ključne riječi (engleski): Anastomosis, Surgical; Animals; Anti-Inflammatory Agents / pharmacology; Anti-Inflammatory Agents / therapeutic use; Anti-Ulcer Agents / pharmacology; Anti-Ulcer Agents / therapeutic use; Ataxia / drug therapy; Behavior, Animal / drug effects; Brain / pathology; Brain Injuries / chemically induced; Brain Injuries / drug therapy; Brain Injuries / pathology; Brain Injuries / physiopathology; Colitis, Ulcerative / chemically induced; Colitis, Ulcerative / drug therapy; Colitis, Ulcerative / pathology; Colitis, Ulcerative / physiopathology; Colon / pathology; Colon / surgery; Cuprizone; Cysteamine; Forelimb / physiopathology; Inflammatory Bowel Diseases / drug therapy; Male; Multiple Sclerosis / drug therapy; Neuroprotective Agents / pharmacology; Neuroprotective Agents / therapeutic use; Peptide Fragments / pharmacology; Peptide Fragments / therapeutic use; Proteins / pharmacology; Proteins / therapeutic use; Rats; Rats, Wistar
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Journal of Physiology and Pharmacology
• Brojčani podaci: vol. 64, br. 5, str. 597-612
• p-ISSN: 0867-5910
• URN:NBN: urn:nbn:hr:105:280120
• Datum objave publikacije: 2013-10
• URL dokumenta: http://www.jpp.krakow.pl/journal/archive/10_13/pdf/597_10_13_article.pdf
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-27 20:01:29