prikaz prve stranice dokumenta Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
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Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
Croatian Medical Journal, 54 (2013), 5; 489-495. https://doi.org/10.3325/cmj.2013.54.489
Mužić, Vedrana; Katušić Bojanac, Ana; Jurić-Lekić, Gordana; Himelreich, Marta; Tupek, Katarina; Šerman, Ljiljana; Marn, Nina; Sinčić, Nino; Vlahović, Maja; Bulić-Jakuš, Floriana

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Mužić, V., Katušić Bojanac, A., Jurić Lekić, G., Himelreich, M., Tupek, K., Šerman, Lj. ... Bulić Jakuš, F. (2013). Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro. Croatian Medical Journal, 54. (5), 489-495. doi: 10.3325/cmj.2013.54.489

Mužić, Vedrana, et al. "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro." Croatian Medical Journal, vol. 54, br. 5, 2013, str. 489-495. https://doi.org/10.3325/cmj.2013.54.489

Mužić, Vedrana, Ana Katušić Bojanac, Gordana Jurić Lekić, Marta Himelreich, Katarina Tupek, Ljiljana Šerman, Nina Marn, Nino Sinčić, Maja Vlahović i Floriana Bulić Jakuš. "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro." Croatian Medical Journal 54, br. 5 (2013): 489-495. https://doi.org/10.3325/cmj.2013.54.489

Mužić, V., et al. (2013) 'Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro', Croatian Medical Journal, 54(5), str. 489-495. doi: 10.3325/cmj.2013.54.489

Mužić V, Katušić Bojanac A, Jurić Lekić G, Himelreich M, Tupek K, Šerman Lj, i sur.. Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro. Croatian Medical Journal [Internet]. 28.10.2013. [pristupljeno 14.11.2024.];54(5):489-495. doi: 10.3325/cmj.2013.54.489

V. Mužić, et al., "Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro", Croatian Medical Journal, vol. 54, br. 5, str. 489-495, Listopad 2013. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:251611. [Citirano: 14.11.2024.]

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Podaci o radu
Naslov (engleski)Epigenetic drug 5-azacytidine impairs proliferation of rat limb buds in an organotypic model-system in vitro
AutorVedrana Mužić
AutorAna Katušić Bojanac
AutorGordana Jurić-Lekić
AutorMarta Himelreich
AutorKatarina Tupek
AutorLjiljana Šerman
AutorNina Marn
AutorNino Sinčić
AutorMaja Vlahović
AutorFloriana Bulić-Jakuš
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
AIM: To establish an organotypic in vitro model of limb bud development to verify whether epigenetic drug and teratogen 5-azacytidine (5azaC) has an effect on limb buds independent of its effects on the placenta. ----- METHODS: Fischer strain rat fore- and hindlimb buds were microsurgically isolated from 13 days old embryos and cultivated in vitro for two weeks at the air-liquid interface in Eagle's minimum essential medium (MEM) with 50% rat serum. 30 μmol of 5azaC was added to the fresh medium. Overall growth was measured by an ocular micrometer. Routine histology, immunohistochemical detection of the proliferating cell nuclear antigen (PCNA), and stereological quantification of PCNA expression were performed. ----- RESULTS: At four time points, significantly lower overall growth was detected for fore- and hindlimb bud explants cultivated with 5azaC in comparison to controls. After the culture period, numerical density of the PCNA signal for both types of limb buds was lower than for controls (P<0.001). Limb buds were initially covered by immature epithelium and contained mesenchyme, myotubes, single hemangioblasts, hemangioblast aggregates, blood islands, and capillaries. Regardless of the treatment, cartilage and epidermis differentiated, but cells and structures typical for vasculogenesis disappeared. ----- CONCLUSION: Our findings, obtained outside of the maternal organism, stress the importance of compromised cell proliferation for 5azaC impact on limb buds. This investigation points to the necessity to establish alternatives to in vivo research on animals using teratogenic agents.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian Medical Journal
Brojčani podacivol. 54, br. 5, str. 489-495
p-ISSN0353-9504
DOIhttps://doi.org/10.3325/cmj.2013.54.489
URN:NBNurn:nbn:hr:105:251611
Datum objave publikacije2013-10-28
URL dokumentahttps://doi.org/10.3325/cmj.2013.54.489
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-27 20:07:11