Antinociceptive effect of botulinum toxin type A on experimental abdominal pain

Znanstveni rad - Izvorni znanstveni rad

• Autor: Višnja Drinovac
• Autor: Lidija Bach-Rojecky
• Autor: Ana Babić
• Autor: Zdravko Lacković
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti

Sažetak (engleski)

Visceral pain, especially in the abdominal region, represents one of the most common types of pain. Its chronic form is usually very hard to treat by conventional analgesic agents and adjuvants. We investigated the antinociceptive effect of botulinum toxin type A (BTX-A) in male Wistar rats in two models of visceral pain: peritonitis induced by intraperitoneal injection of 1% acetic acid and colitis induced by intracolonic instillation of 0.1% capsaicin. Pain was measured as the number of abdominal writhes. Additionally, referred mechanical sensitivity in the ventral abdominal area was evaluated by von Frey test and the extent of spinal c-Fos expression was immunohistochemically examined. BTX-A significantly reduced the number of abdominal writhes in both models of visceral pain after intrathecal application in a dose of 2 U/kg. In the experimental colitis model, BTX-A (2 U/kg) reduced both referred mechanical allodynia and c-Fos expression in the dorsal horn of the spinal cord (S2/S3 segments). In contrast to intrathecal administration, BTX-A (2 U/kg) administered into the cisterna magna had no effect on pain suggesting that the primary site of its action is a spinal cord.

• Ključne riječi (engleski): Abdominal Pain/drug therapy; Abdominal Pain/etiology; Abdominal Pain/physiopathology; Acetic Acid/toxicity; Analgesics/administration & dosage; Analgesics/therapeutic use; Animals; Botulinum Toxins, Type A/administration & dosage; Botulinum Toxins, Type A/therapeutic use; Capsaicin/toxicity; Colitis/complications; Colitis/physiopathology; Disease Models, Animal; Hyperalgesia/drug therapy; Hyperalgesia/etiology; Hyperalgesia/physiopathology; Male; Pain Measurement; Pain, Referred/drug therapy; Pain, Referred/etiology; Pain, Referred/physiopathology; Peritonitis/complications; Peritonitis/physiopathology; Proto-Oncogene Proteins c-fos/metabolism; Rats; Rats, Wistar; Spinal Cord/drug effects; Spinal Cord/physiopathology; Visceral Pain/drug therapy; Visceral Pain/etiology; Visceral Pain/physiopathology
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Završna verzija rukopisa prihvaćena za objavljivanje (postprint)
• Naslov časopisa: European Journal of Pharmacology
• Brojčani podaci: vol. 745, str. 190-195
• p-ISSN: 0014-2999
• DOI: https://doi.org/10.1016/j.ejphar.2014.10.038
• URN:NBN: urn:nbn:hr:105:724295
• Datum objave publikacije: 2014-12-15
• URL dokumenta: https://doi.org/10.1016/j.ejphar.2014.10.038
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-27 20:15:51