prikaz prve stranice dokumenta Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment
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Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment
Experimental Hematology, 42 (2014), 11; 966-975. https://doi.org/10.1016/j.exphem.2014.07.262
Kuzmac, Sania; Grčević, Danka; Šućur, Alan; Ivčević, Sanja; Katavić, Vedran

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Kuzmac, S., Grčević, D., Šućur, A., Ivčević, S. i Katavić, V. (2014). Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment. Experimental Hematology, 42. (11), 966-975. doi: 10.1016/j.exphem.2014.07.262

Kuzmac, Sania, et al. "Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment." Experimental Hematology, vol. 42, br. 11, 2014, str. 966-975. https://doi.org/10.1016/j.exphem.2014.07.262

Kuzmac, Sania, Danka Grčević, Alan Šućur, Sanja Ivčević i Vedran Katavić. "Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment." Experimental Hematology 42, br. 11 (2014): 966-975. https://doi.org/10.1016/j.exphem.2014.07.262

Kuzmac, S., et al. (2014) 'Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment', Experimental Hematology, 42(11), str. 966-975. doi: 10.1016/j.exphem.2014.07.262

Kuzmac S, Grčević D, Šućur A, Ivčević S, Katavić V. Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment. Experimental Hematology [Internet]. 2014. [pristupljeno 11.04.2024.];42(11):966-975. doi: 10.1016/j.exphem.2014.07.262

S. Kuzmac, D. Grčević, A. Šućur, S. Ivčević i V. Katavić, "Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment", Experimental Hematology, vol. 42, br. 11, str. 966-975, 2014. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:868980. [Citirano: 11.04.2024.]

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Naslov (engleski)Acute hematopoietic stress in mice is followed by enhanced osteoclast maturation in the bone marrow microenvironment
AutorSania Kuzmac
AutorDanka Grčević
AutorAlan Šućur
AutorSanja Ivčević
AutorVedran Katavić
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Osteoclasts are components of hematopoietic stem cell (HSC) niches, but their role as contributors to the HSC homeostasis and release are still controversial. We aimed to investigate whether an acute blood loss of 10% of total blood content, along with the consequent intense hematopoiesis, would affect osteoclast differentiation and activity. Isolated peripheral blood, spleen, and bone marrow (BM) cells from bones of hind limbs were investigated for the presence of specific subpopulations of osteoclast precursors: B220(-)CD3(-)NK1.1(-)CD11b(-/low)CD115(+)CD117(+) cells in BM, and B220(-)CD3(-)NK1.1(-)Gr-1(-)CD11b(+)CD115(+) cells in peripheral blood and spleen as well as the receptor activator of nuclear factor κ-B(+) cycle-arrested quiescent osteoclast precursors. Expression of osteoclastogenesis-related genes CD115, receptor activator of nuclear factor κ-B, and cathepsin K, the potential of BM cells to form osteoclast-like cells in vitro, and osteoclast activity in vivo were also evaluated. We observed an increase in spleen cellularity and myelopoiesis during week 1 following blood loss, without any significant effects on BM cellularity or BM myeloid precursors, including cells with high osteoclastogenic potential. However, at 1 week postbleeding, hematopoiesis significantly promoted the expression of cathepsin K, interleukin-34, and bone morphogenetic protein-6. Quiescent osteoclast precursors increased significantly in spleen 2 days following bleeding, whereas osteoclast activity remained unchanged up to 2 weeks postbleeding. Osteoclast-dependent B-cell differentiation was affected at the pre-B stage of maturation in BM, whereas the Lin(-)Sca-1(+)c-kit(+) population expanded in BM and spleen after 2 days postbleeding. Our data demonstrate that an acute blood loss promotes differentiation and maturation of osteoclasts at 1 week but does not enhance osteoresorption at 2 weeks postbleeding. Our data also identify osteoclast differentiation as a consequent and important event in establishing HSC homeostasis following hematopoietic stress.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaExperimental Hematology
Brojčani podacivol. 42, br. 11, str. 966-975
p-ISSN0301-472X
DOIhttps://doi.org/10.1016/j.exphem.2014.07.262
URN:NBNurn:nbn:hr:105:868980
Datum objave publikacije2014
URL dokumentahttps://doi.org/10.1016/j.exphem.2014.07.262
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-27 20:18:29