prikaz prve stranice dokumenta Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
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Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
International Journal of Molecular Sciences, 15 (2014), 6; 10635-10651. https://doi.org/10.3390/ijms150610635
Kafka, Anja; Tomas, Davor; Beroš, Vili; Pećina, Hrvoje Ivan; Zeljko, Martina; Pećina-Šlaus, Nives

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Kafka, A., Tomas, D., Beroš, V., Pećina, H. I., Zeljko, M. i Pećina Šlaus, N. (2014). Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin. International Journal of Molecular Sciences, 15. (6), 10635-10651. doi: 10.3390/ijms150610635

Kafka, Anja, et al. "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin." International Journal of Molecular Sciences, vol. 15, br. 6, 2014, str. 10635-10651. https://doi.org/10.3390/ijms150610635

Kafka, Anja, Davor Tomas, Vili Beroš, Hrvoje Ivan Pećina, Martina Zeljko i Nives Pećina Šlaus. "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin." International Journal of Molecular Sciences 15, br. 6 (2014): 10635-10651. https://doi.org/10.3390/ijms150610635

Kafka, A., et al. (2014) 'Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin', International Journal of Molecular Sciences, 15(6), str. 10635-10651. doi: 10.3390/ijms150610635

Kafka A, Tomas D, Beroš V, Pećina HI, Zeljko M, Pećina Šlaus N. Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin. International Journal of Molecular Sciences [Internet]. 13.06.2014. [pristupljeno 09.11.2024.];15(6):10635-10651. doi: 10.3390/ijms150610635

A. Kafka, D. Tomas, V. Beroš, H. I. Pećina, M. Zeljko i N. Pećina Šlaus, "Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin", International Journal of Molecular Sciences, vol. 15, br. 6, str. 10635-10651, Lipanj 2014. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:707303. [Citirano: 09.11.2024.]

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Podaci o radu
Naslov (engleski)Brain metastases from lung cancer show increased expression of DVL1, DVL3 and beta-catenin and down-regulation of E-cadherin
AutorAnja Kafka
AutorDavor Tomas
AutorVili Beroš
AutorHrvoje Ivan Pećina
AutorMartina Zeljko
AutorNives Pećina-Šlaus
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
The susceptibility of brain to secondary formation from lung cancer primaries is a well-known phenomenon. In contrast, the molecular basis for invasion and metastasis to the brain is largely unknown. In the present study, 31 brain metastases that originated from primary lung carcinomas were analyzed regarding over expression of Dishevelled-1 (DVL1), Dishevelled-3 (DVL3), E-cadherin (CDH1) and beta-catenin (CTNNB1). Protein expressions and localizations were analyzed by immunohistochemistry. Genetic alterations of E-cadherin were tested by polymerase chain reaction (PCR)/loss of heterozygosity (LOH). Heteroduplex was used to investigate mutations in beta-catenin. DVL1 and DVL3 showed over expression in brain metastasis in 87.1% and 90.3% of samples respectively. Nuclear staining was observed in 54.8% of cases for DVL1 and 53.3% for DVL3. The main effector of the Wnt signaling, beta-catenin, was up-regulated in 56%, and transferred to the nucleus in 36% of metastases. When DVL1 and DVL3 were up-regulated the number of cases with nuclear beta-catenin significantly increased (p=0.0001). Down-regulation of E-cadherin was observed in 80% of samples. Genetic analysis showed 36% of samples with LOH of the CDH1. In comparison to other lung cancer pathologies, the diagnoses adenocarcinoma and small cell lung cancer (SCLC) were significantly associated to CDH1 LOH (p=0.001). Microsatellite instability was detected in one metastasis from adenocarcinoma. Exon 3 of beta-catenin was not targeted. Altered expression of Dishevelled-1, Dishevelled-3, E-cadherin and beta-catenin were present in brain metastases which indicates that Wnt signaling is important and may contribute to better understanding of genetic profile conditioning lung cancer metastasis to the brain.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaInternational Journal of Molecular Sciences
Brojčani podacivol. 15, br. 6, str. 10635-10651
p-ISSN1422-0067
DOIhttps://doi.org/10.3390/ijms150610635
URN:NBNurn:nbn:hr:105:707303
Datum objave publikacije2014-06-13
URL dokumentahttp://doi.org/10.3390/ijms150610635
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 08:07:05