prikaz prve stranice dokumenta Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
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Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
International Orthopaedics, 37 (2013), 3; 529-541. https://doi.org/10.1007/s00264-012-1714-3
Brkljačić, Jelena; Pauk, Martina; Erjavec, Igor; Cipčić, Antonio; Grgurević, Lovorka; Zadro, Renata; Inman, Gareth J.; Vukičević, Slobodan

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Brkljačić, J., Pauk, M., Erjavec, I., Cipčić, A., Grgurević, L., Zadro, R. ... Vukičević, S. (2013). Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity. International Orthopaedics, 37. (3), 529-541. doi: 10.1007/s00264-012-1714-3

Brkljačić, Jelena, et al. "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity." International Orthopaedics, vol. 37, br. 3, 2013, str. 529-541. https://doi.org/10.1007/s00264-012-1714-3

Brkljačić, Jelena, Martina Pauk, Igor Erjavec, Antonio Cipčić, Lovorka Grgurević, Renata Zadro, Gareth J. Inman i Slobodan Vukičević. "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity." International Orthopaedics 37, br. 3 (2013): 529-541. https://doi.org/10.1007/s00264-012-1714-3

Brkljačić, J., et al. (2013) 'Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity', International Orthopaedics, 37(3), str. 529-541. doi: 10.1007/s00264-012-1714-3

Brkljačić J, Pauk M, Erjavec I, Cipčić A, Grgurević L, Zadro R, i sur.. Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity. International Orthopaedics [Internet]. 03.2013. [pristupljeno 18.10.2024.];37(3):529-541. doi: 10.1007/s00264-012-1714-3

J. Brkljačić, et al., "Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity", International Orthopaedics, vol. 37, br. 3, str. 529-541, Ožujak 2013. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:842682. [Citirano: 18.10.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:842682
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Podaci o radu
Naslov (engleski)Exogenous heparin binds and inhibits bone morphogenetic protein 6 biological activity
AutorJelena Brkljačić
AutorMartina Pauk
AutorIgor Erjavec
AutorAntonio Cipčić
AutorLovorka Grgurević
AutorRenata Zadro
AutorGareth J. Inman
AutorSlobodan Vukičević
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
PURPOSE: The purpose of this study was to explore the effect of heparin on bone morphogenetic protein 6 (BMP6) osteogenic activity. ----- METHODS: Western blot analysis was used to confirm the binding of BMP6 to heparin and to observe its effect on BMP6 signaling in C2C12-BRE-Luc myoblasts. Real-time RT-PCR was performed for the expression analysis of alkaline phosphatase (ALP) and osteocalcin (OC) in C2C12 myoblasts treated with BMP6 and heparin for 72 hours. Rat ectopic bone formation assay was performed to explore the effect of heparin on BMP6 osteogenic activity. Two weeks following implantation the implants were analysed morphologically and histologically. A mouse osteoporotic model was used to test the ability of BMP6 to improve the bone quality in vivo in the presence of heparin, followed by DEXA and μCT analyses. Blood coagulation was tested in rats previously treated with BMP6. ----- RESULTS: BMP6 specifically bound to heparin and induced Smad1/5/8 phosphorylation which was inhibited by heparin. After 48 and 72 hours of treatment, heparin inhibited BMP6-induced ALP and OC expression in C2C12 cells. Heparin dose dependently inhibited BMP6-induced new bone and cartilage formation in the rat ectopic bone formation assay, while in osteoporotic mice heparin inhibited the BMP6 potential to improve the bone quality as evidenced by decreased bone mineral density and trabecular bone parameters. Interestingly, BMP6 prevented the effect of heparin on the blood coagulation parameters. ----- CONCLUSION: The interaction of BMP6 with heparin might contribute to the heparin-induced osteoporosis and blood coagulation.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaInternational Orthopaedics
Brojčani podacivol. 37, br. 3, str. 529-541
p-ISSN0341-2695
DOIhttps://doi.org/10.1007/s00264-012-1714-3
URN:NBNurn:nbn:hr:105:842682
Datum objave publikacije2013-03
URL dokumentahttp://doi.org/10.1007/s00264-012-1714-3
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 08:09:19