Hepatitis E in patients with hepatic disorders and HIV-infected patients in Croatia: is one diagnostic method enough for hepatitis E diagnosis?

Znanstveni rad - Izvorni znanstveni rad

• Autor: Oktavija Đaković Rode
• Autor: Lorena Jemeršić
• Autor: Dragan Brnić
• Autor: Nenad Pandak
• Autor: Radojka Mikulić
• Autor: Josip Begovac
• Autor: Adriana Vince
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti

Sažetak (engleski)

We assessed hepatitis E virus (HEV) seroprevalence in patients with hepatic disorders as well as in human immunodeficiency virus (HIV)-infected patients and emphasised the issue of possible non-specific anti-HEV seroresponse and need for combining diagnostic methods for hepatitis E diagnosis. Over a two-year period, from March 2011 to February 2013, we determined anti-HEV immunoglobulin M (IgM) and IgG by enzyme immunoassays (EIA; Mikrogen, Germany) in 504 hepatitis patients negative for acute viral hepatitis A-C. Furthermore, 88 samples from randomly selected consecutive HIV-infected patients were also analysed. All EIA reactive samples were additionally tested by line immunoblot assays (LIA; Mikrogen, Germany). HEV nested reverse transcription polymerase chain reaction (RT-PCR) was carried out in 14 anti-HEV IgM LIA-positive patients. Anti-HEV IgM or IgG were detected in 16.9 % of patients by EIA and confirmed by LIA in 10.7 % [95 % confidence interval (CI) 8.3-13.7 %] of hepatitis patients. HEV RNA was detected in five patients. The agreement between EIA and LIA assessed by Cohen's kappa was 0.47 (95 % CI 0.55-0.75) for IgM and 0.83 (95 % CI 0.78-0.93) for IgG. Anti-HEV IgM and IgG seroprevalence in HIV-infected patients was 1.1 %, respectively. Our findings show a rather high HEV seroprevalence in patients with elevated liver enzymes in comparison to HIV-infected patients. Discordant findings by different methods stress the need to combine complementary methods and use a two-tier approach with prudent interpretation of reactive serological results for hepatitis E diagnosis.

• Ključne riječi (engleski): Adolescent; Adult; Aged; Aged, 80 and over; Croatia/epidemiology; Female; HIV Infections/blood; HIV Infections/epidemiology; HIV Infections/virology; Hepatitis Antibodies/blood; Hepatitis E/blood; Hepatitis E/diagnosis; Hepatitis E/epidemiology; Hepatitis E/immunology; Hepatitis E/virology; Humans; Male; Middle Aged; Seroepidemiologic Studies; Young Adult
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Završna verzija rukopisa prihvaćena za objavljivanje (postprint)
• Naslov časopisa: European Journal of Clinical Microbiology & Infectious Diseases
• Brojčani podaci: vol. 33, br. 12, str. 2231-2236
• p-ISSN: 0934-9723
• DOI: https://doi.org/10.1007/s10096-014-2187-7
• URN:NBN: urn:nbn:hr:105:284149
• Datum objave publikacije: 2014-12
• URL dokumenta: https://doi.org/10.1007/s10096-014-2187-7
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 08:09:30