Immunohistochemical analysis of the expression of MAGE-A and NY-ESO-1 cancer/testis antigens in diffuse large B-cell testicular lymphoma

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• Autor: Tvrtko Hudolin
• Autor: Željko Kaštelan
• Autor: Ivana Ilić
• Autor: Katarina Levarda-Hudolin
• Autor: Nikolina Bašić-Jukić
• Autor: Malte Rieken
• Autor: Giulio C. Spagnoli
• Autor: Antonio Juretić
• Autor: Chantal Mengus
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti

Sažetak (engleski)

BACKGROUND:

Primary testicular lymphoma (PTL) is a rare and lethal disease. The most common histological subtype is diffuse large B-cell lymphoma (DLBCL). Standard treatments are frequently ineffective. Thus, the development of novel forms of therapy is urgently required. Specific immunotherapy generating immune responses directed against antigen predominantly expressed by cancer cells such as cancer-testis antigens (CTA) may provide a valid alternative treatment for patients bearing PTL, alone or in combination with current therapies. ----- METHODS:

Three monoclonal antibodies (mAbs), 77B recognizing MAGE-A1, 57B recognizing an epitope shared by multiple MAGE-A CTA (multi-MAGE-A specific) and D8.38 recognizing NY-ESO-1/LAGE-1 were used for immunohistochemical staining of 27 PTL, including 24 DLBCL. ----- RESULTS:

Expression of MAGE-A1 was infrequently detectable in DLBCL specimens (12.50%), whereas multi-MAGE-A and NY-ESO-1/LAGE-1 specific reagents stained the cytoplasms of tumor cells in DLBCL specimens with higher frequencies (54.17% and 37.50%, respectively) with different expression levels. ----- CONCLUSIONS:

These results suggest that MAGE-A and NY-ESO-1/LAGE-1, possibly in combination with other CTA, might be used as targets for specific immunotherapy in DLBCL.

• Ključne riječi (engleski): Antibodies, Monoclonal/chemistry; Antigens, Neoplasm/metabolism; Cytoplasm/metabolism; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Immunotherapy; Lymphoma, B-Cell/metabolism; Lymphoma, Large B-Cell, Diffuse/metabolism; Male; Melanoma-Specific Antigens/metabolism; Membrane Proteins/metabolism; Testicular Neoplasms/metabolism; Testis/metabolism
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Journal of Translational Medicine
• Brojčani podaci: vol. 11, str. 123
• p-ISSN: 1479-5876
• DOI: https://doi.org/10.1186/1479-5876-11-123
• URN:NBN: urn:nbn:hr:105:632408
• Datum objave publikacije: 2013-05-16
• URL dokumenta: http://doi.org/10.1186/1479-5876-11-123
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 08:12:02