prikaz prve stranice dokumenta Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer
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Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer
PLoS ONE, 10 (2015), 6; e0129452. https://doi.org/10.1371/journal.pone.0129452
Milutin Gašperov, Nina; Sabol, Ivan; Planinić, Pavao; Grubišić, Goran; Fistonić, Ivan; Ćorušić, Ante; Grce, Magdalena

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Milutin Gašperov, N., Sabol, I., Planinić, P., Grubišić, G., Fistonić, I., Ćorušić, A. i Grce, M. (2015). Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer. PLoS ONE, 10. (6). doi: 10.1371/journal.pone.0129452

Milutin Gašperov, Nina, et al. "Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer." PLoS ONE, vol. 10, br. 6, 2015. https://doi.org/10.1371/journal.pone.0129452

Milutin Gašperov, Nina, Ivan Sabol, Pavao Planinić, Goran Grubišić, Ivan Fistonić, Ante Ćorušić i Magdalena Grce. "Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer." PLoS ONE 10, br. 6 (2015). https://doi.org/10.1371/journal.pone.0129452

Milutin Gašperov, N., et al. (2015) 'Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer', PLoS ONE, 10(6). doi: 10.1371/journal.pone.0129452

Milutin Gašperov N, Sabol I, Planinić P, Grubišić G, Fistonić I, Ćorušić A, i sur.. Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer. PLoS ONE [Internet]. 09.06.2015. [pristupljeno 05.05.2024.];10(6). doi: 10.1371/journal.pone.0129452

N. Milutin Gašperov, et al., "Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer", PLoS ONE, vol. 10, br. 6, Lipanj 2015. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:378145. [Citirano: 05.05.2024.]

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Podaci o radu
Naslov (engleski)Methylated host cell gene promoters and human papillomavirus type 16 and 18 predicting cervical lesions and cancer
AutorNina Milutin Gašperov
AutorIvan Sabol
AutorPavao Planinić
AutorGoran Grubišić
AutorIvan Fistonić
AutorAnte Ćorušić
AutorMagdalena Grce
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Change in the host and/or human papillomavirus (HPV) DNA methylation profile is probably one of the main factors responsible for the malignant progression of cervical lesions to cancer. To investigate those changes we studied 173 cervical samples with different grades of cervical lesion, from normal to cervical cancer. The methylation status of nine cellular gene promoters, CCNA1, CDH1, C13ORF18, DAPK1, HIC1, RARβ2, hTERT1, hTERT2 and TWIST1, was investigated by Methylation Specific Polymerase Chain Reaction (MSP). The methylation of HPV18 L1-gene was also investigated by MSP, while the methylated cytosines within four regions, L1, 5'LCR, enhancer, and promoter of the HPV16 genome covering 19 CpG sites were evaluated by bisulfite sequencing. Statistically significant methylation biomarkers distinguishing between cervical precursor lesions from normal cervix were primarily C13ORF18 and secondly CCNA1, and those distinguishing cervical cancer from normal or cervical precursor lesions were CCNA1, C13ORF18, hTERT1, hTERT2 and TWIST1. In addition, the methylation analysis of individual CpG sites of the HPV16 genome in different sample groups, notably the 7455 and 7694 sites, proved to be more important than the overall methylation frequency. The majority of HPV18 positive samples contained both methylated and unmethylated L1 gene, and samples with L1-gene methylated forms alone had better prognosis when correlated with the host cell gene promoters' methylation profiles. In conclusion, both cellular and viral methylation biomarkers should be used for monitoring cervical lesion progression to prevent invasive cervical cancer.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaPLoS ONE
Brojčani podacivol. 10, br. 6, e0129452
p-ISSN1932-6203
DOIhttps://doi.org/10.1371/journal.pone.0129452
URN:NBNurn:nbn:hr:105:378145
Datum objave publikacije2015-06-09
URL dokumentahttps://doi.org/10.1371/journal.pone.0129452
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 08:29:54