prikaz prve stranice dokumenta Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
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Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
Journal of Virological Methods, 135 (2006), 2; 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013
Kozić, Sanja; Vince, Adriana; Iščić Beš, Janja; Đaković Rode, Oktavija; Židovec Lepej, Snježana; Poljak, Mario; Bozic, Michael; Kessler, Harald H.

Citirajte ovaj rad

Kozić, S., Vince, A., Iščić Beš, J., Đaković Rode, O., Židovec Lepej, S., Poljak, M. ... Kessler, H. H. (2006). Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients. Journal of Virological Methods, 135. (2), 263-268. doi: 10.1016/j.jviromet.2006.03.013

Kozić, Sanja, et al. "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients." Journal of Virological Methods, vol. 135, br. 2, 2006, str. 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013

Kozić, Sanja, Adriana Vince, Janja Iščić Beš, Oktavija Đaković Rode, Snježana Židovec Lepej, Mario Poljak, Michael Bozic i Harald H. Kessler. "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients." Journal of Virological Methods 135, br. 2 (2006): 263-268. https://doi.org/10.1016/j.jviromet.2006.03.013

Kozić, S., et al. (2006) 'Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients', Journal of Virological Methods, 135(2), str. 263-268. doi: 10.1016/j.jviromet.2006.03.013

Kozić S, Vince A, Iščić Beš J, Đaković Rode O, Židovec Lepej S, Poljak M, i sur.. Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients. Journal of Virological Methods [Internet]. 2006. [pristupljeno 30.12.2024.];135(2):263-268. doi: 10.1016/j.jviromet.2006.03.013

S. Kozić, et al., "Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients", Journal of Virological Methods, vol. 135, br. 2, str. 263-268, 2006. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:744800. [Citirano: 30.12.2024.]

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Podaci o radu
Naslov (engleski)Evaluation of a commercial real-time PCR assay for quantitation of Epstein-Barr virus DNA in different groups of patients
AutorSanja Kozić
AutorAdriana Vince
AutorJanja Iščić Beš
AutorOktavija Đaković Rode
AutorSnježana Židovec Lepej
AutorMario Poljak
AutorMichael Bozic
AutorHarald H. Kessler
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
The aim of this study was to evaluate the performance of a molecular assay for quantitation of Epstein-Barr virus (EBV) DNA based on real-time PCR, and to determine EBV DNA levels in EDTA whole blood samples derived from different groups of patients. Following a manual DNA extraction protocol, real-time PCR was performed using the LightCycler EBV Quantification Kit, which demonstrated sufficient accuracy and linearity. Coefficients of variations were found to be between 6 and 42% and 5 and 34%, respectively, for inter-assay and intra-assay variations. In clinical specimens, EBV DNA was detected in all patients with acute EBV infection (n=34), in one of 25 adults with past EBV infection, in 16 out of 25 (64%) anti-HIV antibody positive persons, in 10 out of 25 (40%) solid organ transplant recipients, and in none of the 23 infants without history of EBV infection. When EBV DNA levels in positive specimens were compared between different groups, statistically significant differences were not found. The LightCycler EBV Quantification Kit was found to be useful for determination of EBV DNA levels in EDTA whole blood.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaJournal of Virological Methods
Brojčani podacivol. 135, br. 2, str. 263-268
p-ISSN0166-0934
DOIhttps://doi.org/10.1016/j.jviromet.2006.03.013
URN:NBNurn:nbn:hr:105:744800
Datum objave publikacije2006
URL dokumentahttps://doi.org/10.1016/j.jviromet.2006.03.013
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 08:59:12