prikaz prve stranice dokumenta Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
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Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
Croatian Medical Journal, 59 (2018), 5; 213-223. https://doi.org/10.3325/cmj.2018.59.213
Kafka, Anja; Karin-Kujundžić, Valentina; Šerman, Ljiljana; Bukovac, Anja; Njirić, Niko; Jakovčević, Antonia; Pećina-Šlaus, Nives

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Kafka, A., Karin Kujundžić, V., Šerman, Lj., Bukovac, A., Njirić, N., Jakovčević, A. i Pećina Šlaus, N. (2018). Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades. Croatian Medical Journal, 59. (5), 213-223. doi: 10.3325/cmj.2018.59.213

Kafka, Anja, et al. "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades." Croatian Medical Journal, vol. 59, br. 5, 2018, str. 213-223. https://doi.org/10.3325/cmj.2018.59.213

Kafka, Anja, Valentina Karin Kujundžić, Ljiljana Šerman, Anja Bukovac, Niko Njirić, Antonia Jakovčević i Nives Pećina Šlaus. "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades." Croatian Medical Journal 59, br. 5 (2018): 213-223. https://doi.org/10.3325/cmj.2018.59.213

Kafka, A., et al. (2018) 'Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades', Croatian Medical Journal, 59(5), str. 213-223. doi: 10.3325/cmj.2018.59.213

Kafka A, Karin Kujundžić V, Šerman Lj, Bukovac A, Njirić N, Jakovčević A, i sur.. Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades. Croatian Medical Journal [Internet]. 31.10.2018. [pristupljeno 24.12.2024.];59(5):213-223. doi: 10.3325/cmj.2018.59.213

A. Kafka, et al., "Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades", Croatian Medical Journal, vol. 59, br. 5, str. 213-223, Listopad 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:300029. [Citirano: 24.12.2024.]

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Podaci o radu
Naslov (engleski)Hypermethylation of Secreted Frizzled Related Protein 1 gene promoter in different astrocytoma grades
AutorAnja Kafka
AutorValentina Karin-Kujundžić
AutorLjiljana Šerman
AutorAnja Bukovac
AutorNiko Njirić
AutorAntonia Jakovčević
AutorNives Pećina-Šlaus
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Aim: To identify the involvement of Secreted Frizzled Related Protein 1 (SFRP1) promoter hypermethylation in different malignancy grades of astrocytoma and assess its association with beta-catenin, lymphoid-enhancer factor 1, and T-cell factor 1. Methods: Twenty-six astrocytoma samples were collected from 2008-2015. Promoter hypermethylation was evaluated by methylation-specific polymerase-chain-reaction and protein expression by immunohistochemistry and stereological analysis. The staining intensity was scored by comparing immunoreactivity with normal tissue and by using 10% and 50% cut-offs. Results: SFRP1 promoter methylation was found in 32% of astrocytomas. The number of hypermethylated samples increased in higher astrocytoma grades and was the highest in glioblastoma (P = 0.042 compared to other astrocytoma grades). There was 45.8% of samples with the lack of or weak expression of SFRP1 protein and 29.2% with strong expression. Samples with methylated promoter expressed significantly less SFRP1 than samples with unmethylated promoter (P = 0.031). Beta-catenin expression levels were elevated. Yet, glioblastomas with unmethylated SFRP1 promoter had significantly less beta-catenin (P = 0.033). Strong expression of lymphoid-enhancer factor 1 was associated to higher astrocytoma grades (P = 0.006). Conclusion: SFRP1 gene was epigenetically silenced in glioblastomas when compared to low astrocytoma grades, which may suggest that the lack of its protein is involved in astrocytoma progression.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaCroatian Medical Journal
Brojčani podacivol. 59, br. 5, str. 213-223
p-ISSN0353-9504
DOIhttps://doi.org/10.3325/cmj.2018.59.213
URN:NBNurn:nbn:hr:105:300029
Datum objave publikacije2018-10-31
URL dokumentahttps://doi.org/10.3325/cmj.2018.59.213
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 09:47:56