Transplantation of neural stem cells in the mouse model of ischemic brain stroke and expression of genes involved in programmed cell death

Znanstveni rad - Izvorni znanstveni rad

• Autor: Valentina Hribljan
• Autor: Iva Salamon
• Autor: Arijana Đemaili
• Autor: Ivan Alić
• Autor: Dinko Mitrečić
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Temeljne medicinske znanosti

Sažetak (engleski)

Aim: To analyze how neural stem cells (NSC) transplantation in the stroke-affected mouse brain influences the expression of genes involved in apoptosis-inducing factor (AIF)-mediated cell death – apoptosis inducing factor mitochondria associated 1 (Aifm1), ring finger protein 146 (Rnf146, Iduna), and cyclophilin A (CypA); necroptosis –receptor interaction protein kinase 1 (Ripk1), Ripk3, and mixed-lineage kinase domain-like protein (Mlkl); and apoptosis – Caspase 3 (Casp3) and Casp8. Methods: Four groups of animals were used to obtain mRNA for quantitative reverse transcription polymerase chain reaction analysis: healthy animals (n = 3), animals with stroke (n = 4), animals with stroke treated by stem cell transplantation (n = 7), and animals with stroke treated by proliferation-supporting medium (n = 5). Ischemic brain injury was induced by transient left middle cerebral artery occlusion. Statistical analysis was performed using one-way analysis of variance with post-hoc Tukey test. Results: NSC transplantation in the stroke-affected mouse brain significantly increased the expression of Iduna (P < 0.05), a gene-encoding protein with well-known protective effects on hypoxic damage, and significantly down-regulated the expression of damage-supportive genes, Casp3 (P < .01) and Aifm1 (P < 0.001). We were able to distinguish between the effect produced by stem cell transplantation (Iduna, Aifm1, Ripk3, Mlkl) and the effect produced by supporting the tissue with proliferation-supporting medium (Ripk1, Casp8). Conclusion: Beside revealing some clearly positive effects of stem cells transplantation on the stroke-affected brain, our results suggest that the tissue response triggered by stem cells points toward the desired, regeneration-supporting levels of expression of a certain gene at a certain time point.

• Ključne riječi (engleski): Animals; Apoptosis/genetics; Apoptosis Inducing Factor/genetics; Brain Ischemia/genetics; Brain Ischemia/therapy; Caspases/genetics; Cell Differentiation; Cyclophilin A/genetics; Disease Models, Animal; Female; Gene Expression Regulation/physiology; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; Neural Stem Cells/transplantation; Protein Kinases/genetics; RNA, Messenger/genetics; Receptor-Interacting Protein Serine-Threonine Kinases/genetics; Reverse Transcriptase Polymerase Chain Reaction; Stem Cell Transplantation; Stroke/genetics; Stroke/therapy; Ubiquitin-Protein Ligases/genetics
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Croatian Medical Journal
• Brojčani podaci: vol. 59, br. 5, str. 203-212
• p-ISSN: 0353-9504
• DOI: https://doi.org/10.3325/cmj.2018.59.203
• URN:NBN: urn:nbn:hr:105:323762
• Datum objave publikacije: 2018-10-31
• URL dokumenta: https://doi.org/10.3325/cmj.2018.59.203
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 09:50:31