Influence of hyperthermal regimes on experimental teratoma development in vitro

Znanstveni rad - Izvorni znanstveni rad

• Autor: Ana Katušić Bojanac
• Autor: Srđan Rogošić
• Autor: Nino Sinčić
• Autor: Gordana Jurić-Lekić
• Autor: Maja Vlahovic
• Autor: Ljiljana Šerman
• Autor: Davor Ježek
• Autor: Floriana Bulić-Jakuš
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti

Sažetak (engleski)

We screened for the impact of hyperthermal regimes varying in the cumulative
equivalent minutes at 43°C (CEM43°C) and media composition on tumour development
using an original teratoma in vitro model. Rat embryos (three germ layers)
were microsurgically isolated and cultivated at the air-liquid interface. During
a two week period, ectodermal, mesodermal and endodermal derivatives developed
within trilaminar teratomas. Controls were grown at 37°C. Overall growth
was measured, and teratoma survival and differentiation were histologically
assessed. Cell proliferation was stereologically quantified by the volume density
of Proliferating Cell Nuclear Antigen. Hyperthermia of 42°C, applied for 15 minutes
after plating (CEM43°C 3.75 minutes), diminished cell proliferation
(P ˂ .0001) and enhanced differentiation of both myotubes (P ˂ .01) and cylindrical
epithelium (P ˂ .05). Hyperthermia of 43°C applied each day for 30 minutes
during the first week (CEM43°C 210 minutes) impaired overall growth
(P ˂ .01) and diminished cell proliferation (P ˂ .0001). Long-term hyperthermia
of 40.5°C applied for two weeks (CEM43°C 630 minutes) significantly impaired
survival (P ˂ .005). Long-term hyperthermia of 40.5°C applied from the second
day when differentiation of tissues begins (CEM43°C 585 minutes) impaired survival
(P ˂ .0001), overall growth (P ˂ .01) and cartilage differentiation
(P ˂ .05). No teratomas survived extreme regimes: 43°C for 24 hours (CEM43°C
1440 minutes), hyperthermia in the scant serum-free medium (CEM43°C
630 minutes) or treatment with an anti-HSP70 antibody before long-term hyperthermia
40.5°C from the second day (CEM43°C 585 minutes). This in vitro
research provided novel insights into the impact of hyperthermia on the development
of experimental teratomas from their undifferentiated sources and are thus
of potential interest for future therapeutic strategies in corresponding in vivo
models.

• Ključne riječi (engleski): Animals; Cell Differentiation; Cell Proliferation; Cell Survival; Embryo Culture Techniques; Embryo, Mammalian/metabolism; Embryo, Mammalian/pathology; Female; Hyperthermia, Induced/methods; Pregnancy; Proliferating Cell Nuclear Antigen/metabolism; Rats, Inbred F344; Teratoma/metabolism; Teratoma/pathology; Teratoma/prevention & control; Time Factors
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: International Journal of Experimental Pathology
• Brojčani podaci: vol. 99, br. 3, str. 131-144
• p-ISSN: 1365-2613
• DOI: https://doi.org/10.1111/iep.12273
• URN:NBN: urn:nbn:hr:105:178759
• Datum objave publikacije: 2018-06
• URL dokumenta: https://doi.org/10.1111/iep.12273
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2024-03-28 10:06:42