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PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants
Molecular and Cellular Probes, 22 (2008), 2; 110-114. https://doi.org/10.1016/j.mcp.2007.10.003
Mitrečić, Dinko; Mavrić, Sandra; Gajović, Srećko

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Mitrečić, D., Mavrić, S. i Gajović, S. (2008). PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants. Molecular and Cellular Probes, 22. (2), 110-114. doi: 10.1016/j.mcp.2007.10.003

Mitrečić, Dinko, et al. "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants." Molecular and Cellular Probes, vol. 22, br. 2, 2008, str. 110-114. https://doi.org/10.1016/j.mcp.2007.10.003

Mitrečić, Dinko, Sandra Mavrić i Srećko Gajović. "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants." Molecular and Cellular Probes 22, br. 2 (2008): 110-114. https://doi.org/10.1016/j.mcp.2007.10.003

Mitrečić, D., Mavrić, S. i Gajović, S. (2008) 'PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants', Molecular and Cellular Probes, 22(2), str. 110-114. doi: 10.1016/j.mcp.2007.10.003

Mitrečić D, Mavrić S, Gajović S. PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants. Molecular and Cellular Probes [Internet]. 04.2008. [pristupljeno 08.11.2024.];22(2):110-114. doi: 10.1016/j.mcp.2007.10.003

D. Mitrečić, S. Mavrić i S. Gajović, "PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants", Molecular and Cellular Probes, vol. 22, br. 2, str. 110-114, Travanj 2008. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:472380. [Citirano: 08.11.2024.]

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Naslov (engleski)PCR-based identification of short deletion/insertions and single nucleotide substitutions in genotyping of splotch (Pax3(sp)) and truncate (Noto(tc)) mouse mutants
AutorDinko Mitrečić
AutorSandra Mavrić
AutorSrećko Gajović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Splotch (Pax3(sp)) and truncate (Noto(tc)) are spontaneously arisen mouse mutants with disturbed embryo development. Splotch carries a Pax3 mutation and it is characterized by the neural tube defect. Corresponding mutation in human causes Waardenburg syndrome. Truncate is Noto mutant with disturbed development of the caudal notochord. In order to establish easy genotyping procedure of these mutations, it was tested whether simple PCRs with single primer pairs could be used for this purpose. As it was necessary to differentiate sequence variants on the scale of one to several nucleotides, the approach referred to as "3' variable primer ends" was applied. The method was based on the presence of discriminating nucleotides at the 3' end of the primer sequence. This approach was successfully applied in genotyping adult mice and embryos of splotch with a 6bp deletion/insertion and truncate with a single nucleotide substitution. Described genotyping approach facilitates recognizing of these mutations and it could be in general used for detection of sequence differences in one to several nucleotides.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaMolecular and Cellular Probes
Brojčani podacivol. 22, br. 2, str. 110-114
p-ISSN0890-8508
DOIhttps://doi.org/10.1016/j.mcp.2007.10.003
URN:NBNurn:nbn:hr:105:472380
Datum objave publikacije2008-04
URL dokumentahttps://doi.org/10.1016/j.mcp.2007.10.003
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2024-03-28 10:11:38