prikaz prve stranice dokumenta A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic
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A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic
Stem Cells and Development, 28 (2019), 11; 717-733. https://doi.org/10.1089/scd.2018.0194
Sobočan, Nikola; Katušić Bojanac, Ana; Sinčić, Nino; Himelreich-Perić, Marta; Krasić, Jure; Majić, Željka; Jurić-Lekić, Gordana; Šerman, Ljiljana; Vlahović, Maja; Ježek, Davor; Bulić-Jakuš, Floriana

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Sobočan, N., Katušić Bojanac, A., Sinčić, N., Himelreich Perić, M., Krasić, J., Majić, Ž. ... Bulić Jakuš, F. (2019). A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic. Stem Cells and Development, 28. (11), 717-733. doi: 10.1089/scd.2018.0194

Sobočan, Nikola, et al. "A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic." Stem Cells and Development, vol. 28, br. 11, 2019, str. 717-733. https://doi.org/10.1089/scd.2018.0194

Sobočan, Nikola, Ana Katušić Bojanac, Nino Sinčić, Marta Himelreich Perić, Jure Krasić, Željka Majić, Gordana Jurić Lekić, et al. "A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic." Stem Cells and Development 28, br. 11 (2019): 717-733. https://doi.org/10.1089/scd.2018.0194

Sobočan, N., et al. (2019) 'A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic', Stem Cells and Development, 28(11), str. 717-733. doi: 10.1089/scd.2018.0194

Sobočan N, Katušić Bojanac A, Sinčić N, Himelreich Perić M, Krasić J, Majić Ž, i sur.. A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic. Stem Cells and Development [Internet]. 01.06.2019. [pristupljeno 20.04.2024.];28(11):717-733. doi: 10.1089/scd.2018.0194

N. Sobočan, et al., "A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic", Stem Cells and Development, vol. 28, br. 11, str. 717-733, Lipanj 2019. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:278242. [Citirano: 20.04.2024.]

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Naslov (engleski)A free radical scavenger ameliorates teratogenic activity of a DNA hypomethylating hematological therapeutic
AutorNikola Sobočan
AutorAna Katušić Bojanac
AutorNino Sinčić
AutorMarta Himelreich-Perić
AutorJure Krasić
AutorŽeljka Majić
AutorGordana Jurić-Lekić
AutorLjiljana Šerman
AutorMaja Vlahović
AutorDavor Ježek
AutorFloriana Bulić-Jakuš
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
The spin-trap free radical scavenger N-tert-butyl-a-phenylnitron (PBN) ameliorated effects of several teratogens involving reactive oxygen species (ROS). We investigated for the first time whether PBN could ameliorate teratogenesis induced by a DNA hypomethylating hematological therapeutic 5-azacytidine (5azaC). At days 12 and 13 of gestation, Fisher rat dams were pretreated by an i.v. injection of PBN (40mg/kg) and 1 h later by an i.p. injection of 5azaC (5mg/kg). Development was analyzed at gestation day 15 in embryos and day 20 in fetuses. PBN alone did not significantly affect development. PBN pretreatment restored survival of 5azaC-treated dams’ embryos to the control level, restored weight of embryos and partially of fetuses, and partially restored crownrump lengths. PBN pretreatment converted limb adactyly to less severe oligodactyly. PBN pretreatment restored global DNA methylation level in the limb buds to the control level. Cell proliferation in limb buds of all 5azaCtreated dams remained significantly lower than in controls. In the embryonic liver, PBN pretreatment normalized proliferation diminished significantly by 5azaC; whereas in embryonic vertebral cartilage, proliferation of all 5azaC-treated dams was significantly higher than in PBN-treated dams or controls. Apoptotic indices significantly enhanced by 5azaC in liver and cartilage were not influenced by PBN pretreatment. However, PBN significantly diminished ROS or reactive nitrogen species markers nitrotyrosine and 8-hydroxy-2¢deoxyguanosine elevated by 5azaC in embryonic tissues, and, therefore, activity of this DNA hypomethylating agent was associated to the activation of free radicals. That pretreatment with PBN enhanced proliferation in the liver and not in immature tissue is interesting for the treatment of 5azaC-induced hepatotoxicity and liver regeneration.
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaStem Cells and Development
Brojčani podacivol. 28, br. 11, str. 717-733
p-ISSN1547-3287
DOIhttps://doi.org/10.1089/scd.2018.0194
URN:NBNurn:nbn:hr:105:278242
Datum objave publikacije2019-06-01
URL dokumentahttp://doi.org/10.1089/scd.2018.0194
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 10:30:21