prikaz prve stranice dokumenta Mice genotyping using buccal swab samples: an improved method
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Mice genotyping using buccal swab samples: an improved method
Biochemical Genetics, 46 (2008), 3-4; 105-112. https://doi.org/10.1007/s10528-007-9133-7
Mitrečić, Dinko; Mavrić, Sandra; Vrabec Branica, Božica; Gajović, Srećko

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Mitrečić, D., Mavrić, S., Vrabec Branica, B. i Gajović, S. (2008). Mice genotyping using buccal swab samples: an improved method. Biochemical Genetics, 46. (3-4), 105-112. doi: 10.1007/s10528-007-9133-7

Mitrečić, Dinko, et al. "Mice genotyping using buccal swab samples: an improved method." Biochemical Genetics, vol. 46, br. 3-4, 2008, str. 105-112. https://doi.org/10.1007/s10528-007-9133-7

Mitrečić, Dinko, Sandra Mavrić, Božica Vrabec Branica i Srećko Gajović. "Mice genotyping using buccal swab samples: an improved method." Biochemical Genetics 46, br. 3-4 (2008): 105-112. https://doi.org/10.1007/s10528-007-9133-7

Mitrečić, D., et al. (2008) 'Mice genotyping using buccal swab samples: an improved method', Biochemical Genetics, 46(3-4), str. 105-112. doi: 10.1007/s10528-007-9133-7

Mitrečić D, Mavrić S, Vrabec Branica B, Gajović S. Mice genotyping using buccal swab samples: an improved method. Biochemical Genetics [Internet]. 04.2008. [pristupljeno 19.10.2024.];46(3-4):105-112. doi: 10.1007/s10528-007-9133-7

D. Mitrečić, S. Mavrić, B. Vrabec Branica i S. Gajović, "Mice genotyping using buccal swab samples: an improved method", Biochemical Genetics, vol. 46, br. 3-4, str. 105-112, Travanj 2008. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:869960. [Citirano: 19.10.2024.]

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Naslov (engleski)Mice genotyping using buccal swab samples: an improved method
AutorDinko Mitrečić
AutorSandra Mavrić
AutorBožica Vrabec Branica
AutorSrećko Gajović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Routine methods used to genotype mice involve isolation of DNA from partially amputated neonate's tail, toe, or ear. The inevitable drawbacks of such techniques are the animal's pain response and the increased time and funds required for DNA purification. In order to implement a noninvasive and simple protocol for mouse DNA isolation, we have improved the method based on samples collected by swabbing of the inner cheek. Combining alkaline and temperature lysis, it was possible to isolate a DNA solution ready for PCR in less than an hour. Testing the method on three different mouse lines showed that it is highly efficient, the volume of the PCR samples could be reduced to 25 microl, and fragments up to 800 bp were successfully amplified. This protocol reduces animal discomfort, shortens the time for DNA isolation, and enables amplification of larger DNA fragments with optimal success rate, thus considerably facilitating large-scale genotyping of different mouse lines.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeZavršna verzija rukopisa prihvaćena za objavljivanje (postprint)
Naslov časopisaBiochemical Genetics
Brojčani podacivol. 46, br. 3-4, str. 105-112
p-ISSN0006-2928
DOIhttps://doi.org/10.1007/s10528-007-9133-7
URN:NBNurn:nbn:hr:105:869960
Datum objave publikacije2008-04
URL dokumentahttp://doi.org/10.1007/s10528-007-9133-7
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2024-03-28 11:32:23