Utjecaj povišene i snižene razine perifernog serotonina na trabekularnu koštanu masu štakora

master's thesis

• Title (english): The influence of raised and reduced levels of peripheral serotonin on trabecular bone mass in rats
• Author: Mia Rora
• Mentor: Slobodan Vukičević (mentor)
• Committee member: Lovorka Grgurević (predsjednik povjerenstva)
• Committee member: Zdravko Petanjek (član povjerenstva)
• Committee member: Slobodan Vukičević (član povjerenstva)
• Granter: University of Zagreb; School of Medicine; (Department of Anatomy and Clinical Anatomy); Zagreb
• Defense date and country: 2015-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: BIOMEDICINE AND HEALTHCARE; Basic Medical Sciences; Anatomy

Abstract

U nedavnim istraţivanjima se pokazalo kako periferni serotonin djeluje inhibitorno na proces koštane formacije. U našem istraţivanju smo koristili sublinije ţivotinja dobivene selektivnim kriţanjem sa višom odnosno niţom razinom perifernog serotonina što predstavlja fiziološki model za razliku od modela s izbaĉenim genom u kojem su dobivene ekstremne vrijednosti. Kako bi pokazali utjecaj perifernog i centralnog serotonina analizirali smo distalni dio bedrene kosti i kraljeţnicu mikro CT-om te denzitometrijom. PotvrĊene su statistiĉki znaĉajne razlike relevantnih koštanih parametara ţivotinja sa sniţenom razinom serotonina u odnosu na ţivotinje sa povišenom razinom serotonina. Porastom vrijednosti serotonina dolazi do sniţavanja trabekularnog koštanog volumena. Nadalje, ţivotinje sa povišenom razinom serotonina imale su veću duljinu bedrene i goljeniĉne kosti od ţivotinja sa sniţenom razinom serotonina. Dobiveni rezultati mogli bi posluţiti u daljnjim istraţivanjima i razvoju nove anaboliĉke terapije kojoj bi mehanizam djelovanja bio inhibicija kljuĉnih dijelova serotoninskog puta.

Abstract (english)

Recent studies have shown that peripheral serotonin has an inhibitory effect on the process of bone formation. In our research, we used sublines of animals that have developed, through selective cross-breeding higher and lower levels of peripheral serotonin, which represents a physiological model, as opposed to the model with the excluded gene in which extreme values were obtained. To show the effect of peripheral and central serotonin, we have analysed the distal femur and the spine using micro-CT and densitometry. We confirmed statistically significant differences in relevant bone parameters of animals with reduced levels of serotonin, compared to animals with raised levels. The increase in the serotonin value leads to lowering trabecular bone volume. Measuring length and width of femur and shin bone we have found that increasing serotonin values leads to increasing bone length and width compared to animals with reduced levels of serotonin. The acquired results could help further research and the development of a new anabolic therapy, whose mechanism of action would be the inhibition of key parts of serotonergic pathways.

• Keywords: serotonin; kost; anaboliĉka terapija
• Keywords (english): serotonin; bone; anabolic therapy
• Language: croatian
• URN:NBN: urn:nbn:hr:105:618857
• Study programme: Title: Medicine; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: doktor/doktorica medicine (doktor/doktorica medicine)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2016-08-12 08:30:48