Unique miRNA Expression Profile in MSI- and EMAST-Unstable Sporadic Colon Cancer

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• Autor: Sonja Marinović
• Autor: Kristina Vuković Đerfi
• Autor: Anita Škrtić
• Autor: Mirko Poljak
• Autor: Sanja Kapitanović
• Autorova ustanova: Sveučilište u Zagrebu; Medicinski fakultet; (KATEDRA ZA PATOLOGIJU)
• Znanstveno / umjetničko područje, polje i grana: BIOMEDICINA I ZDRAVSTVO; Kliničke medicinske znanosti; Patologija

Sažetak (engleski)

MicroRNAs (miRNAs) are critical post-transcriptional gene regulators and their involvement in sporadic colon cancer (CRC) tumorigenesis has been confirmed. In this study we investigated differences in miRNA expression in microsatellite stable (MSS/EMAST-S), microsatellite unstable marked by high elevated microsatellite alterations at selected tetranucleotide repeats (MSS/EMAST-H), and high microsatellite unstable (MSI-H/EMAST-H) tumor subgroups as well as in tumors with different clinicopathologic characteristics. An RT-qPCR analysis of miRNA expression was carried out on 45 colon cancer and adjacent normal tissue samples (15 of each group). Overall, we found three differentially expressed miRNAs between the subgroups. miR-92a-3p and miR-224-5p were significantly downregulated in MSI-H/EMAST-H tumors in comparison to other subgroups. miR-518c-3p was significantly upregulated in MSS/EMAST-H tumors in comparison to stable and highly unstable tumors. Furthermore, we showed that miR-143-3p and miR-145-5p were downregulated in tumors in comparison to normal tissues in all subgroups. In addition, we showed overexpression of miR-125b-5p in well-differentiated tumors and miR-451a in less advanced tumors. This is the first report on differences in miRNA expression profiles between MSS/EMAST-S, MSS/EMAST-H, and MSI-H/EMAST-H colorectal cancers. Our findings indicate that the miRNA expression signatures differ in CRC subgroups based on their instability status.

• Ključne riječi (engleski): Aged; Colonic Neoplasms / genetics; Colonic Neoplasms / pathology; Female; Gene Expression Profiling / methods; Gene Expression Regulation, Neoplastic; Humans; Male; MicroRNAs / genetics; Microsatellite Instability; Microsatellite Repeats / genetics; Middle Aged; Transcriptome / genetics
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Genes
• Brojčani podaci: vol. 15, br. 8, 1007
• e-ISSN: 2073-4425
• DOI: https://doi.org/10.3390/genes15081007
• URN:NBN: urn:nbn:hr:105:743821
• Datum objave publikacije: 2024-08-01
• Projekt: Šifra: HRZZ-IP-2016-06-1430; Naziv (hrvatski): Mikrosatelitna nestabilnost (MSI i EMAST) u molekularnom profiliranju sporadičnih karcinoma debelog crijeva; Naziv (engleski): Microsatellite instability (MSI and EMAST) in molecular profiling of sporadic colon cancers; Kratica: MSIandEMASTinCRC; Pravna nadležnost: Hrvatska; Financijer: Hrvatska zaklada za znanost; Linija financiranja: Research Projects
• URL dokumenta: https://www.mdpi.com/2073-4425/15/8/1007
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Datum i vrijeme pohrane: 2025-03-25 11:43:19